Coding

Part:BBa_K3841010:Design

Designed by: Emil Ørsted Christensen   Group: iGEM21_DTU-Denmark   (2021-09-02)


Particulate methane monooxygenase alpha subunit (PmoB)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 619
    Illegal XbaI site found at 1153
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 334
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 619
    Illegal XbaI site found at 1153
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 619
    Illegal XbaI site found at 1153
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used GeneWiz to codon optimize the sequence for expression in Komagataella phaffii. Additionally, we removed any BsaI and BsmBI sites within the coding sequence.



Source

https://www.uniprot.org/uniprot/G1UBD1

References